广州医大团队:粪便放室温一定时间,菌群有何变化?
  • 样品储存条件对于微生物群落宏基因组的无偏差分析十分重要;
  • 收集29名健康婴儿的粪便样品,每个样品分为5份分别置于室温储存不同时间后冻于-80;
  • 样品中的微生物群落的α多样性不受室温储存时间的影响;
  • 样品中的微生物组成及UniFrac距离也不受室温影响。
#取样和DNA提取方法集#
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蓝灿辉 | 热心肠先生
把婴儿粪便样本室温放置2小时内,会对菌群分析有影响么?看看广州医科大学团队的研究结果。
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Scientific Reports [IF:3.998]

Effect of short-term room temperature storage on the microbial community in infant fecal samples

短时间室温储存对婴儿粪便样品中微生物群落的影响

10.1038/srep26648

2016-05-26, Article

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Sample storage conditions are important for unbiased analysis of microbial communities in metagenomic studies. Specifically, for infant gut microbiota studies, stool specimens are often exposed to room temperature (RT) conditions prior to analysis. This could lead to variations in structural and quantitative assessment of bacterial communities. To estimate such effects of RT storage, we collected feces from 29 healthy infants (0-3 months) and partitioned each sample into 5 portions to be stored for different lengths of time at RT before freezing at -80 °C. Alpha diversity did not differ between samples with storage time from 0 to 2 hours. The UniFrac distances and microbial composition analysis showed significant differences by testing among individuals, but not by testing between different time points at RT. Changes in the relative abundance of some specific (less common, minor) taxa were still found during storage at room temperature. Our results support previous studies in children and adults, and provided useful information for accurate characterization of infant gut microbiomes. In particular, our study furnished a solid foundation and justification for using fecal samples exposed to RT for less than 2 hours for comparative analyses between various medical conditions.

First Authors:
Yong Guo,Sheng-Hui Li,Ya-Shu Kuang

Correspondence Authors:
HongWen Deng,Xiu Qiu

All Authors:
Yong Guo,Sheng-Hui Li,Ya-Shu Kuang,Jian-Rong He,Jin-Hua Lu,Bei-Jun Luo,Feng-Ju Jiang,Yao-Zhong Liu,Christopher J Papasian,Hui-Min Xia,HongWen Deng,Xiu Qiu

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