用光遗传学定量、定时控制肠菌代谢,促进宿主长寿
创作:白蓝木 审核:爱的抉择 01月08日
  • 开发了一种利用光遗传学控制秀丽隐杆线虫肠道中细菌的基因表达和代谢产物产生的方法;
  • 对大肠杆菌菌株进行基因改造,利用光可以定量控制荚膜异多糖酸(CA)的分泌;
  • 使用这种由光遗传学控制的菌株在秀丽隐杆线虫肠道中诱导CA的产生,保护线虫肠道细胞的线粒体免受应激引起的过度断裂的影响;
  • 该菌株的寿命延长效应与绿光强度呈正相关,表明宿主的寿命在与CA存在剂量效应;
  • 光遗传学可用于实现肠道细菌代谢的定量和时间控制。
主编推荐语
爱的抉择
肠道微生物代谢与宿主寿命有关,但在原位控制微生物代谢建立两者之间的因果关系,目前仍然是具有挑战性的。eLife近期发表的文章,开发了利用光遗传学控制肠道细菌基因表达的系统,同时在秀丽隐杆线虫肠道的大肠杆菌中进行实践,验证了光控制的荚膜异多糖酸的产生可保护肠道细胞线粒体。研究证明了光遗传学可用于肠道菌群代谢的定量和定时控制,便于研究其对宿主健康和衰老的局部和系统性影响。
关键字
延伸阅读本研究的原文信息和链接出处,以及相关解读和评论文章。欢迎读者朋友们推荐!
图片
eLife [IF:7.08]

Optogenetic control of gut bacterial metabolism to promote longevity

光遗传学控制肠道细菌代谢以促进长寿

10.7554/eLife.56849

2020-12-16, Article

Abstract & Authors:展开

Abstract:收起
Gut microbial metabolism is associated with host longevity. However, because it requires direct manipulation of microbial metabolism in situ, establishing a causal link between these two processes remains challenging. We demonstrate an optogenetic method to control gene expression and metabolite production from bacteria residing in the host gut. We genetically engineer an Escherichia coli strain that secretes colanic acid (CA) under the quantitative control of light. Using this optogenetically-controlled strain to induce CA production directly in the Caenorhabditis elegans gut, we reveal the local effect of CA in protecting intestinal mitochondria from stress-induced hyper-fragmentation. We also demonstrate that the lifespan-extending effect of this strain is positively correlated with the intensity of green light, indicating a dose-dependent CA benefit on the host. Thus, optogenetics can be used to achieve quantitative and temporal control of gut bacterial metabolism in order to reveal its local and systemic effects on host health and aging.

First Authors:
Lucas A Hartsough

Correspondence Authors:
Meng C Wang,Jeffrey J Tabor

All Authors:
Lucas A Hartsough,Mooncheol Park,Matthew V Kotlajich,John Tyler Lazar,Bing Han,Chih-Chun J Lin,Elena Musteata,Lauren Gambill,Meng C Wang,Jeffrey J Tabor

图片
Bio生物世界微信公众号

eLife:光遗传学新突破,调控肠道微生物,延长寿命

生物世界,2020-12-25

评论