仝小林+李敏+王军:葛根芩连汤调节肠道菌群,缓解大鼠糖尿病
创作:徐硕 审核:mildbreeze 2021年10月22日
  • 分别使用葛根芩连汤(GQD)、二甲双胍(Met)或生理盐水治疗2型糖尿病大鼠12周,观测血糖、肠道屏障和炎症水平,分析肠道菌群;
  • GQD和Met均改善大鼠血糖、HbA1c、HOMA-IR和OGTT血糖水平;
  • GQD改善结肠occludin等肠道屏障指标表达水平,降低了大鼠CRP、内毒素等炎症指标表达水平;
  • GQD改善糖尿病大鼠肠道菌群多样性,升高Flavonifractor等有益菌丰度,降低Butyricimonas等条件致病菌丰度,且部分菌属和糖尿病相关生化指标密切相关。
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mildbreeze
葛根芩连汤(GQD)是一种中药,有报道称它能显著降低血糖水平,可能与肠道菌群有关。中国中医科学院广安门医院仝小林院士、李敏和中科院微生物所王军与团队,近期在Frontiers in cellular and infection microbiology发表研究,揭示了GQD对糖尿病大鼠的肠道菌群、肠屏障功能和炎症的影响,表明GQD的降糖机制可能是由其对肠道菌群的调控所介导的。
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Alleviation Effects of GQD, a Traditional Chinese Medicine Formula, on Diabetes Rats Linked to Modulation of the Gut Microbiome

中药方剂葛根芩连汤对糖尿病大鼠的缓解作用与肠道菌群调节有关

10.3389/fcimb.2021.740236

2021-10-08, Article

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Gegen Qinlian Decoction (GQD) is a Chinese herbal medicine that has been reported to significantly decrease blood glucose levels, which is suggested to be related to interactions with the gut microbiota. However, the protective effect of GQD on intestinal barrier function with regard to its influence on the gut microbiota has not been explored to date. In this study, we investigated the role of the gut microbiota in mediating the hypoglycemic mechanism of GQD in type 2 diabetes mellitus (T2DM) rats induced by a single intraperitoneal injection of streptozotocin after 4 weeks of high-fat diet feeding. The T2DM rats were randomly allocated to receive GQD, metformin (Met), or saline for 12 consecutive weeks, and changes in metabolic parameters, intestinal barrier function, and inflammation were investigated. Gut microbiota was analyzed using 16S rRNA gene sequencing from fecal samples, and statistical analyses were performed to correlate microbiota composition with phenotypes of the T2DM rats. GQD administration decreased the levels of blood glucose and inflammatory cytokines, and increased the levels of tight junction proteins. Besides, GQD had a protective effect on islet function, restoring intestinal permeability, and inhibiting inflammation, as evidenced by increases in the levels of serum C-peptide, occludin, and claudin-1 in the colon, and also improved the expression of serum inflammatory factors. In addition, GQD regulated the structure of the gut microbiota by increasing the proportions of short-chain fatty acids-producing and anti-inflammatory bacteria, and decreasing the proportions of conditioned pathogenic bacteria associated with the diabetic phenotype. Overall, these findings suggest that GQD could ameliorate hyperglycemia and protect islet function by regulating the structure of the gut microbiota, thereby restoring intestinal permeability and inhibiting inflammation in T2DM rats. Our study thus suggests that the hypoglycemic mechanism of GQD is mediated by its modulation of the gut microbiota.

First Authors:
Jiaxing Tian,Bingbing Bai

Correspondence Authors:
Jun Wang,Min Li,Xiaolin Tong

All Authors:
Jiaxing Tian,Bingbing Bai,Zezheng Gao,Yingying Yang,Haoran Wu,Xinmiao Wang,Jun Wang,Min Li,Xiaolin Tong

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