中山大学:热量限制或能缓解PM2.5导致的肺损伤
  • 雄性C57BL/6J小鼠饲养在模拟石家庄实际环境PM2.5的暴露系统中4周,用热量限制(CR)饲料或随意(AL)喂养;
  • CR喂养缓解PM所致小鼠肺损伤和肺外毒性,其氧化应激、DNA损伤和炎症减少;
  • PM暴露诱导CR小鼠肺组织中与活性氧、细胞因子产生和炎性细胞活化相关通路的基因显著下调,而介导抗氧化和DNA修复的相关基因被激活;
  • CR小鼠肝脏中异生物质代谢和解毒途径被上调,与体外试验中多环芳烃的尿代谢物水平升高和细胞毒性降低相一致。
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爱的抉择
热量限制可以改善健康,延长寿命。中山大学陈雯团队在《Particle and Fibre Toxicology》发表文章,首次证明热量限制可有效缓解大气颗粒物(PM)暴露诱导的小鼠肺损伤和肺外毒性,并且热量限制激活肝脏的异生物质代谢和增强解毒功能。文章提示,饮食干预可提供治疗手段,减少PM暴露带来的健康风险。
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Caloric restriction attenuates C57BL/6 J mouse lung injury and extra-pulmonary toxicity induced by real ambient particulate matter exposure

热量限制减轻C57BL/6J小鼠因实际环境颗粒物诱导的肺损伤和肺外毒性

10.1186/s12989-020-00354-2

2020-06-05, Other

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Background: Caloric restriction (CR) is known to improve health and extend lifespan in human beings. The effects of CR on adverse health outcomes in response to particulate matter (PM) exposure and the underlying mechanisms have yet to be defined.
Results: Male C57BL/6 J mice were fed with a CR diet or ad libitum (AL) and exposed to PM for 4 weeks in a real-ambient PM exposure system located at Shijiazhuang, China, with a daily mean concentration (95.77 μg/m3) of PM2.5. Compared to AL-fed mice, CR-fed mice showed attenuated PM-induced pulmonary injury and extra-pulmonary toxicity characterized by reduction in oxidative stress, DNA damage and inflammation. RNA sequence analysis revealed that several pulmonary pathways that were involved in production of reactive oxygen species (ROS), cytokine production, and inflammatory cell activation were inactivated, while those mediating antioxidant generation and DNA repair were activated in CR-fed mice upon PM exposure. In addition, transcriptome analysis of murine livers revealed that CR led to induction of xenobiotic metabolism and detoxification pathways, corroborated by increased levels of urinary metabolites of polycyclic aromatic hydrocarbons (PAHs) and decreased cytotoxicity measured in an ex vivo assay.
Conclusion: These novel results demonstrate, for the first time, that CR in mice confers resistance against pulmonary injuries and extra-pulmonary toxicity induced by PM exposure. CR led to activation of xenobiotic metabolism and enhanced detoxification of PM-bound chemicals. These findings provide evidence that dietary intervention may afford therapeutic means to reduce the health risk associated with PM exposure.

First Authors:
Daochuan Li,Shen Chen,Qiong Li

Correspondence Authors:
Wen Chen

All Authors:
Daochuan Li,Shen Chen,Qiong Li,Liping Chen,Haiyan Zhang,Huiyao Li,Dianke Yu,Rong Zhang,Yujie Niu,Shaoyou Lu,Lizhu Ye,Xiaowen Zeng,Guanghui Dong,Rui Chen,Michael Aschner,Yuxin Zheng,Wen Chen

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