抗生素引起恒河猴肠道中的菌群及免疫应答变化
创作:aluba 审核:aluba 2019年12月17日
  • 4种不同抗生素(恩诺沙星、头孢氨苄、巴龙霉素、克林霉素)处理健康雌性恒河猴;
  • 所有抗生素均可破坏恒河猴的肠道菌群,降低螺杆菌科等发酵细菌的丰度,增加肠杆菌科等潜在致病菌的丰度;
  • 肠道菌群组成的变化与短链脂肪酸的减少相关;
  • 抗生素处理改变了结肠粘膜免疫应答,增加中性粒细胞及Th17细胞的数量,并升高了血浆中可溶性CD14的水平,提示菌群易位的增加。
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Mucosal Immunology上发表的一项最新研究,在恒河猴中分析了抗生素处理对肠道菌群及免疫的改变,发现抗生素引起了肠道菌群组成的显著变化,以及结肠粘膜免疫应答的变化。
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Mucosal Immunology [IF:7.313]

Antibiotic-induced microbiome perturbations are associated with significant alterations to colonic mucosal immunity in rhesus macaques

抗生素诱导的菌群变化与恒河猴的结肠粘膜免疫的显著变化相关

10.1038/s41385-019-0238-1

2019-12-04, Article

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The diverse bacterial communities that colonize the gastrointestinal tract play an essential role in maintaining immune homeostasis through the production of critical metabolites such as short-chain fatty acids (SCFAs) and this can be disrupted by antibiotic use. However, few studies have addressed the effects of specific antibiotics longitudinally on the microbiome and immunity. We evaluated the effects of four specific antibiotics: enrofloxacin, cephalexin, paromomycin, and clindamycin, in healthy female rhesus macaques. All antibiotics disrupted the microbiome, including reduced abundances of fermentative bacteria and increased abundances of potentially pathogenic bacteria, including Enterobacteriaceae in the stool, and decreased Helicobacteraceae in the colon. This was associated with decreased SCFAs, indicating altered bacterial metabolism. Importantly, antibiotic use also substantially altered local immune responses, including increased neutrophils and Th17 cells in the colon. Furthermore, we observed increased soluble CD14 in plasma, indicating microbial translocation. These data provide a longitudinal evaluation of antibiotic-induced changes to the composition and function of colonic bacterial communities associated with specific alterations in mucosal and systemic immunity.

First Authors:
Jennifer A Manuzak,Alexander S Zevin

Correspondence Authors:
Nichole R Klatt

All Authors:
Jennifer A Manuzak,Alexander S Zevin,Ryan Cheu,Brian Richardson,Jacob Modesitt,Tiffany Hensley-McBain,Charlene Miller,Andrew T Gustin,Ernesto Coronado,Toni Gott,Mike Fang,Michael Cartwright,Solomon Wangari,Brian Agricola,Drew May,Elise Smith,Hans Benjamin Hampel,Michael Gale,Cheryl M Cameron,Mark J Cameron,Jeremy Smedley,Nichole R Klatt

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