Abstract & Authors:展开
Background: The present study was conducted to investigate the possible association between gut microbes and immunity among healthy middle-aged and elderly individuals in southwest China. A total of 148 healthy adults aged ≥ 50 years were divided into two age groups: middle-aged group (50-59 years; n = 67, 54.13 ± 3.32) and elderly group (≥ 60 years; n = 81, 64.70 ± 3.93). Blood samples were collected to measure serum immune and biochemical indices. Gut microbiota compositions of the groups were characterized on the basis of faecal DNA using 16S rRNA gene sequencing.
Results: Among the detected gut microbes, the presence ofwas negatively correlated with age in both groups. In the middle-aged group, age was negatively correlated with the presence ofand. In the elderly group,was present at significantly higher levels; age was negatively correlated with the presence of,and the Chao index, whereas positively correlated with the presence ofIn the middle-aged group, the presence ofwas positively correlated with serum immunoglobulin G (IgG) levels and the percent of CD8T cells and negatively correlated with the CD4/CD8ratio; the presence ofwas negatively correlated with IgM levels;ratio was positively correlated with IgG and IgM levels and Simpson index was negatively correlated with the percent of CD8T cells and positively correlated with CD4/CD8ratio. In the elderly group, the presence of(identified as genus) was positively correlated with IgA levels and the percent of CD8T cells and negatively correlated with the percent of CD4T cells and CD4/CD8ratio; the Chao index and observed species were positively correlated with IgA levels.
Conclusions: These results indicated that ageing could significantly correlate with the composition of gut microbiota in terms of quantity and quality. Changes in gut microbiota caused by ageing, characterized by decreasedlevels, might be associated with immunosenescence among healthy middle-aged and elderly people in southwest China.
Xi Shen,Junjie Miao
Ming Li,Fang He
Xi Shen,Junjie Miao,Qun Wan,Shuyue Wang,Ming Li,Fangfang Pu,Guoqing Wang,Wei Qian,Qian Yu,Francesco Marotta,Fang He